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intercellular cell adhesion molecule-1 (icam-1) antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc intercellular cell adhesion molecule-1 (icam-1) antibody
    Intercellular Cell Adhesion Molecule 1 (Icam 1) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercellular+cell+adhesion+molecule-1+(icam-1)+antibody/anti+human+icam+1+antibody/10__32604_slash_biocell__2021__012901-49-39-48
    Average 90 stars, based on 1 article reviews
    intercellular cell adhesion molecule-1 (icam-1) antibody - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Betulin inhibits inflammatory factors synthesis in osteoarthritis synovial fibroblasts by suppressing the PI3K, Akt, and mTOR pathways and activating miR-5006-5p
    Article Snippet: The antibodies against mTOR (SC-136369), p-p85 (SC-12929), p85 (SC-1637), p-Akt (SC-16646-R), Akt (SC-5298) and the p85 activator (SC-3036) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). .. The antibodies against ICAM-1 (4915S) and p-mTOR (5536S) were acquired from Cell Signaling (Danvers, MA, USA). .. CHI3L1 antibody (GTX639462) was acquired from GeneTex (Hsinchu, Taiwan).

    Blocking Assay:

    Article Title: Engineering an immune-integrated lung-on-a-chip to reveal TOX-RAGE axis-driven fibrosis and RAGE blockade as a therapeutic strategy.
    Article Snippet: .. Samples were fixed with 4% paraformaldehyde (PFA; P2031, Biosesang, Seongnam, Republic of Korea) for 10 min at room temperature, followed by permeabilization and blocking with 4% bovine serum albumin (BSA) and 0.4% Triton X-100 in PBS for 1 h. After washing, samples were incubated with the following primary antibodies and dyes: α-SMA (14–9760-82, Invitrogen,, 1:500), F-actin (ab176757, Abcam, Cambridge, UK, 1:2000), Fibronectin (#26,836, Cell Signaling Technology, Danvers, MA, USA, 1:500), 5-TAMRA (C6121, Invitrogen, 1:2000 in PBS), VE-cadherin (#2158, Cell Signaling Technology, 1:100), and ICAM-1 (#62,133, Cell Signaling Technology, 1:500). .. Fluorescence images were acquired using a Leica Stellaris confocal microscope (DMI8, Leica Microsystems, Wetzlar, Germany) equipped with 10 × and 25 × objective lenses, operated via Leica Application Suite X (LAS X, Leica Microsystems) software.

    Article Title: Engineering an immune-integrated lung-on-a-chip to reveal TOX–RAGE axis–driven fibrosis and RAGE blockade as a therapeutic strategy
    Article Snippet: .. Samples were fixed with 4% paraformaldehyde (PFA; P2031, Biosesang, Seongnam, Republic of Korea) for 10 min at room temperature, followed by permeabilization and blocking with 4% bovine serum albumin (BSA) and 0.4% Triton X-100 in PBS for 1 h. After washing, samples were incubated with the following primary antibodies and dyes: α-SMA (14–9760-82, Invitrogen,, 1:500), F-actin (ab176757, Abcam, Cambridge, UK, 1:2000), Fibronectin (#26,836, Cell Signaling Technology, Danvers, MA, USA, 1:500), 5-TAMRA (C6121, Invitrogen, 1:2000 in PBS), VE-cadherin (#2158, Cell Signaling Technology, 1:100), and ICAM-1 (#62,133, Cell Signaling Technology, 1:500). .. Fluorescence images were acquired using a Leica Stellaris confocal microscope (DMI8, Leica Microsystems, Wetzlar, Germany) equipped with 10 × and 25 × objective lenses, operated via Leica Application Suite X (LAS X, Leica Microsystems) software.

    Incubation:

    Article Title: Engineering an immune-integrated lung-on-a-chip to reveal TOX-RAGE axis-driven fibrosis and RAGE blockade as a therapeutic strategy.
    Article Snippet: .. Samples were fixed with 4% paraformaldehyde (PFA; P2031, Biosesang, Seongnam, Republic of Korea) for 10 min at room temperature, followed by permeabilization and blocking with 4% bovine serum albumin (BSA) and 0.4% Triton X-100 in PBS for 1 h. After washing, samples were incubated with the following primary antibodies and dyes: α-SMA (14–9760-82, Invitrogen,, 1:500), F-actin (ab176757, Abcam, Cambridge, UK, 1:2000), Fibronectin (#26,836, Cell Signaling Technology, Danvers, MA, USA, 1:500), 5-TAMRA (C6121, Invitrogen, 1:2000 in PBS), VE-cadherin (#2158, Cell Signaling Technology, 1:100), and ICAM-1 (#62,133, Cell Signaling Technology, 1:500). .. Fluorescence images were acquired using a Leica Stellaris confocal microscope (DMI8, Leica Microsystems, Wetzlar, Germany) equipped with 10 × and 25 × objective lenses, operated via Leica Application Suite X (LAS X, Leica Microsystems) software.

    Article Title: Transcriptomic and metabolomic insights into gabapentin's therapeutic role in neurogenic inflammation of rosacea.
    Article Snippet: Proteins were separated by 10% SDS-PAGE, transferred to a PVDF membrane (IPFL00010, Millipore), and blocked with 5% skim milk (P0216, Beyotime) at room temperature for 1 hour. .. The membranes were then incubated overnight at 4°C with diluted primary antibodies: PGP9.5 (MA1-83428, 1/1000, Invitrogen), NF-κB p65 (ab32536, 1/1000, Abcam), ICAM-1 (#4915, 1/1000, CST), VCAM-1 (#32653, 1/2000, CST), IKB-α (ab32518, 1/1000, Abcam), IKKα (ab32041, 1/10000, Abcam), TNF-α (ab183218, 1/1000, Abcam), β-actin (ab8226, 1/1000, Abcam), Histone H1 (PA5-30055, 1/1000, Invitrogen). .. The membranes were then washed thrice with TBST (3×5 minutes) and incubated with AntiRabbit-IgG secondary antibody (7074, 1/1000, CST) or Anti-Mouse-IgG (ab205719, 1/2000, Abcam) at room temperature for 1 hour.

    Article Title: Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosacea
    Article Snippet: Proteins were separated by 10% SDS-PAGE, transferred to a PVDF membrane (IPFL00010, Millipore), and blocked with 5% skim milk (P0216, Beyotime) at room temperature for 1 hour. .. The membranes were then incubated overnight at 4 °C with diluted primary antibodies: PGP9.5 (MA1-83428, 1/1000, Invitrogen), NF-κB p65 (ab32536, 1/1000, Abcam), ICAM-1 (#4915, 1/1000, CST), VCAM-1 (#32653, 1/2000, CST), IKB-α (ab32518, 1/1000, Abcam), IKKα (ab32041, 1/10000, Abcam), TNF-α (ab183218, 1/1000, Abcam), β-actin (ab8226, 1/1000, Abcam), Histone H1 (PA5-30055, 1/1000, Invitrogen). .. The membranes were then washed thrice with TBST (3×5 minutes) and incubated with Anti-Rabbit-IgG secondary antibody (7074, 1/1000, CST) or Anti-Mouse-IgG (ab205719, 1/2000, Abcam) at room temperature for 1 hour.

    Article Title: Engineering an immune-integrated lung-on-a-chip to reveal TOX–RAGE axis–driven fibrosis and RAGE blockade as a therapeutic strategy
    Article Snippet: .. Samples were fixed with 4% paraformaldehyde (PFA; P2031, Biosesang, Seongnam, Republic of Korea) for 10 min at room temperature, followed by permeabilization and blocking with 4% bovine serum albumin (BSA) and 0.4% Triton X-100 in PBS for 1 h. After washing, samples were incubated with the following primary antibodies and dyes: α-SMA (14–9760-82, Invitrogen,, 1:500), F-actin (ab176757, Abcam, Cambridge, UK, 1:2000), Fibronectin (#26,836, Cell Signaling Technology, Danvers, MA, USA, 1:500), 5-TAMRA (C6121, Invitrogen, 1:2000 in PBS), VE-cadherin (#2158, Cell Signaling Technology, 1:100), and ICAM-1 (#62,133, Cell Signaling Technology, 1:500). .. Fluorescence images were acquired using a Leica Stellaris confocal microscope (DMI8, Leica Microsystems, Wetzlar, Germany) equipped with 10 × and 25 × objective lenses, operated via Leica Application Suite X (LAS X, Leica Microsystems) software.

    other:

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis
    Article Snippet: Antibodies for phospho-eIF2α (Ser51, #AF1237), eIF2α (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Virus:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Microarray:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Recombinant:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Chromatin Immunoprecipitation:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Labeling:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Transfection:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Staining:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Viability Assay:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Amplification:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Luciferase:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Reporter Gene Assay:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis

    Real-time Polymerase Chain Reaction:

    Article Title: ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis.
    Article Snippet: Article ICAM1 mRNA entraps ILF2/ILF3 to inhibit transcription of EIF4E and global protein synthesis



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    Cell Signaling Technology Inc intercellular cell adhesion molecule 1 icam 1
    PRDM16 inhibited AS through inactivation of the TGF-β signaling pathway. (A) The content of TC, TG, and HDL-C in peripheral blood in mice in each group as detected by biochemical indicators. (B) The changes of aortic plaques in AS mice as detected by HE staining (×200, scale bar: 50 μm). (C) The number of aortic thrombosis in AS mice as detected by ORO staining (×400, scale bar: 25 μm). (D) Positive rates of AS <t>markers</t> <t>(ICAM-1</t> and VCAM-1) in the aorta of AS mice as detected by IHC (×400). * p < 0.05 compared with mice treated with DMSO, # p < 0.05 compared with mice treated with oe-PRDM16 NC. The measurement data were expressed as mean ± standard deviation, and analyzed by independent-sample t -test, n = 15.
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    Image Search Results


    PRDM16 inhibited AS through inactivation of the TGF-β signaling pathway. (A) The content of TC, TG, and HDL-C in peripheral blood in mice in each group as detected by biochemical indicators. (B) The changes of aortic plaques in AS mice as detected by HE staining (×200, scale bar: 50 μm). (C) The number of aortic thrombosis in AS mice as detected by ORO staining (×400, scale bar: 25 μm). (D) Positive rates of AS markers (ICAM-1 and VCAM-1) in the aorta of AS mice as detected by IHC (×400). * p < 0.05 compared with mice treated with DMSO, # p < 0.05 compared with mice treated with oe-PRDM16 NC. The measurement data were expressed as mean ± standard deviation, and analyzed by independent-sample t -test, n = 15.

    Journal: Frontiers in Physiology

    Article Title: PRDM16 Upregulation Induced by MicroRNA-448 Inhibition Alleviates Atherosclerosis via the TGF-β Signaling Pathway Inactivation

    doi: 10.3389/fphys.2020.00846

    Figure Lengend Snippet: PRDM16 inhibited AS through inactivation of the TGF-β signaling pathway. (A) The content of TC, TG, and HDL-C in peripheral blood in mice in each group as detected by biochemical indicators. (B) The changes of aortic plaques in AS mice as detected by HE staining (×200, scale bar: 50 μm). (C) The number of aortic thrombosis in AS mice as detected by ORO staining (×400, scale bar: 25 μm). (D) Positive rates of AS markers (ICAM-1 and VCAM-1) in the aorta of AS mice as detected by IHC (×400). * p < 0.05 compared with mice treated with DMSO, # p < 0.05 compared with mice treated with oe-PRDM16 NC. The measurement data were expressed as mean ± standard deviation, and analyzed by independent-sample t -test, n = 15.

    Article Snippet: The tissue sections were probed with primary antibodies to intercellular cell adhesion molecule-1 (ICAM-1) (#4915, 1:50, Cell Signaling Technology, Danvers, MA, United States) and vascular cell adhesion molecule-1 (VCAM-1) (ab134047, 1:250, Abcam, Cambridge, United Kingdom) at 4°C overnight.

    Techniques: Staining, Standard Deviation